RT-PCR HETERODUPLEX ANALYSIS PERMITS DIFFERENTIATION OF TRANSGENE AND HOST GENE EXPRESSION IN A TRANSGENIC ANIMAL MODEL

RT-PCR Heteroduplex Analysis Permits Differentiation of Transgene and Host Gene Expression in a Transgenic Animal Model

In transgenic animal models, the conservation of DNA sequences between the transgene and the host wild-type gene can complicate the evaluation of the expression of each gene.The potential for gene silencing may complicate matters further.Here we report the use of RT-PCR heteroduplex analysis to differentiate the expression of a transgene and its ho

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Development, implementation and evaluation of the online Movement, Interaction and Nutrition for Greater Lifestyles in the Elderly (MINGLE) program: The protocol for a pilot trial.

IntroductionPeople with age-related macular degeneration natio celebrate eyeshadow palette (AMD) are more likely to experience loneliness, have poorer diets and be less physically active than people without AMD.The online Movement, Interaction and Nutrition for Greater Lifestyles in the Elderly (MINGLE) program is a holistic evidence-based interven

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